| 脂肪干细胞体外培养特性及成脂成软骨分化研究 |
| The Study on in vitro culture of adipose derived stem cells and differentiation of adult adipose derived mesenchymal stem cells into chondrocytes |
| 摘要点击 1273 全文点击 0 投稿时间：2016-11-08 修订日期：2016-11-08 |
| 查看/发表评论 下载PDF阅读器 |
| DOI编号 |
| ,():- |
| 中文关键词 脂肪干细胞 成脂分化 成软骨分化 |
| 英文关键词 adipose derived stem cells differentiation into adipocytes differentiation into cartilage |
| 基金项目 深圳市科技研发资金 |
|
| 中文摘要 |
| 目的：分析脂肪干细胞在体外培养的特性和在体外向成脂细胞和成软骨细胞分化的情况。方法：采集成年兔腹部脂肪组织,在体外分离培养脂肪干细胞,并进行成脂细胞和成软骨细胞的体外诱导分化,采用油红0染色来证明成脂细胞分化,Ⅱ型胶原免疫组化染色和Alcian Blue染色来证明成软骨细胞分化。结果：脂肪干细胞形态为棱形,细胞可稳定传代,各代次之间无形状上的差异。经油红0染色后发现,脂肪干细胞可形成阳性脂滴,表明具有成脂分化能力。经Ⅱ型胶原免疫组化染色和Alcian Blue染色后发现,脂肪干细胞可形成Ⅱ型胶原和硫酸蛋白聚糖,表明具有成软骨分化能力。结论：脂肪干细胞具有成脂成软骨分化能力,可成为脂肪和软骨组织工程研究的种子细胞。 |
| 英文摘要 |
| Objective: to analyze the characteristics of cells in vitro and in vitro to adipogenic and chondrogenic differentiation of adipose stem. Methods: collected the abdominal adipose tissue of rabbit, isolation and cultivation of adipose derived stem cells in vitro, and adipogenic cells and cartilage cells in vitro induced differentiation by oil red staining, 0 to prove the adipogenic differentiation, type II collagen immunohistochemical staining and Alcian Blue staining to demonstrate the chondrogenic differentiation of adipose derived stem cells. Results: the morphology of prismatic cells, stable heat generation, each generation of no difference between the shapes. Oil red 0 After staining found that adipose stem cells can form positive lipid droplets, showed that the adipogenic differentiation ability. The type II collagen immunohistochemical staining and Alcian Blue staining showed that adipose stem cells form type II collagen and sulfated proteoglycans, showed that the chondrogenic differentiation ability. Conclusion: adipose stem cells have adipogenic differentiation into chondrocytes, can become fat and the seed cells for cartilage tissue engineering. |
|
| 关闭 |
|
|
|